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. 2022 Apr 19;13:2056. doi: 10.1038/s41467-022-29741-3

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© The Author(s) 2022, corrected publication 2024

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Fig. 3 — a Delphilin, labeled with GFP, co-localizes with BFP-labeled Centrin to the basal bodies of MCCs. Actin filaments were visualized by co-expression of RFP-Utrophin. b Fhod1 (GFP-Fhod1) assumes a position in between surrounding actin filaments (phalloidin, red) and basal bodies (BFP-Centrin). c GFP-Fhod3 localizes to the tip of the RFP-Clamp-labeled anterior rootlet. The lower panel shows magnified Clamp- and Fhod3-labeled images. d Fhod3 associates with anterior and posterior rootlets. The posterior rootlets (arrowheads) are oriented orthogonally to the plasma membrane. Depicted are diagonal views after 3D reconstruction of confocal images (IMARIS). Images were obtained at stage 29-30; scale bars, 5 µm).