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. 2022 Apr 19;13:2056. doi: 10.1038/s41467-022-29741-3

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© The Author(s) 2022, corrected publication 2024

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Fig. 4 — a Fhod3 (RFP, red) localizes to the distal end of the anterior rootlets (labeled with BFP-Clamp, blue) in between phalloidin (white)-labeled actin filaments and GFP-labeled FAK (scale bar, 5 µm). Magnified images depict the pair-wise comparison of fluorescent proteins. b The x-z projection reveals the presence of Fhod3 at the distal tip of the anterior basal body rootlet within the subapical actin layer. The left upper image depicts the maximal intensity projection of the confocal section used for 3D reconstruction (scale bar, 1 µm). The right upper picture represents a magnification of the phalloidin (white)-labeled image, depicting the border (dashed line) between apical and subapical (arrowhead) actin layer. While Fhod3 (RFP, red) labeled the tip of the anterior rootlet (BFP-Clamp, blue), FAK (GFP, green) localized to the more proximal part of the rootlet (arrowhead). Magnified images depict the pair-wise comparison of fluorescent proteins. Images were obtained at stage 29-30.