Table 2.

Primers.

Oligonucleotide	Sequence (5′-3′)
For construction of PvraX-gfp fluorescent reporter plasmid:
pCN34_gfp_Fw _vraX	AGCAAAGGAGGTAATATAGGAAAAAAAATGAGTAAAGGAGAAGAACTTTTCACTGG
pCN34_gfp_Rev_vraX	GTTGTATGCACCGTGATCCAGTGTCATCTGAACCATAGGATCCTCT
vraX_Fw	TCCTATGGTTCAGATGACACTGGATCACGGTGCATACAACCG
vraX_rev	CTCCTTTACTCATTTTTTTTCCTATATTACCTCCTTTGCTACTCTATGG
For construction of PdltA-gfp fluorescent reporter plasmid:
pCN34_gfp_Fw_dltA	TCTAATGAGGGAGACTTAATAAAAAAAATGAGTAAAGGAGAAGAAC
pCN34_gfp_Rev_dltA	AATTATCATCAGCGCAAATAGTGTCATCTGAACCATAGGA
dltA_Fw	TCCTATGGTTCAGATGACACTATTTGCGCTGATGATAATTCA
dltA_Rev	TCTCCTTTACTCATTTTTTTTATTAAGTCTCCCTCATTAGAACT
For construction of PvraUTSR complementation plasmid:
pCN34_Fw_vra	TATGTTTTAGAATAGTTACCACAACGTCGTGACTGGGAAA
pCN34_Rev_vra	TAAGTTTTTAATGACTTTCAAAAACGACGGCCAGTGAATT
vraUTSR_Fw	AATTCACTGGCCGTCGTTTTTGAAAGTCATTAAAAACTTAACAGG
vraUTSR_Rev	TTTCCCAGTCACGACGTTGTGGTAACTATTCTAAAACATATGGCA
For construction of PgraXRS complementation plasmid:
pCN34_Fw_gra	AGAAGTTAGATAAAGAACATACAACGTCGTGACTGGGAAA
pCN34_Rev_gra	AAATGTACCACTCAATAACCAAAACGACGGCCAGTGAATT
graXRS_Fw	AATTCACTGGCCGTCGTTTTGGTTATTGAGTGGTACATTTGC
graXRS_Rev	TTTCCCAGTCACGACGTTGTATGTTCTTTATCTAACTTCTGTACC
For construction of PdltD complementation plasmid:
pCN34_Fw_dlt	TATCTTTATAGGCGCCTTTGCGGAAAGAG
pCN34_Rev_dlt	AGCGCAAATAATTCGCCATTCAGGCTGC
dltA_pro_Fw	AATGGCGAATTATTTGCGCTGATGATAATTC
dltA_pro_Rev	TTAATTTCATATTAAGTCTCCCTCATTAGAAC
dltD_Fw	GAGACTTAATATGAAATTAAAACCTTTTTTACCC
dltD_Rev	CAAAGGCGCCTATAAAGATATTAAGTTAACAGAACATATTATG
For construction of Pcls2 complementation plasmid:
pCN34_Fw_cls2	GGCGCCTTTGCGGAAAGAG
pCN34_Rev_cls2	ATTCGCCATTCAGGCTGC
cls2_Fw	AGCCTGAATGGCGAATCTGTTTAACGCCGAACGTG
cls2_Rev	TTCCGCAAAGGCGCCTTTCGTCAACGGTATCATGAAG