Fig. 1. Both CD90⁺49f⁺ and CD90⁻49f⁺ self-renewing populations are interconvertible and give rise to the whole CD34⁺ compartment.

a SRC frequency within the four most primitive populations measured by limiting dilution assay (LDA) at 12 wks post intravenous (i.v) transplantation. 95% confidence interval is shown (see Supplementary Data 1 for details). To obtain unbiased frequencies, similar number of male and female mice were used (Supplementary Fig. 1e). b Cell dose equivalent of ~1 SRC of each population was i.v injected into separate primary NSG recipients (1^ry tx) and human graft was determined at 5 and 12 weeks (wks) later (n = 7–18 mice). Secondary transplantation (2^ry tx) was performed with ~1 secondary SRC dose of CD34⁺CD38^lo/− HSPCs (600 CD45⁺CD34⁺CD38^lo/− cells) derived from CD90⁺CD49f⁺ and CD90⁻CD49f⁺ populations. Mice with sufficient human graft (above the dotted line, see “Methods”) were used for analysis in (c). Each dot represents a mouse (n = 7–12 mice); median lines are shown; results were from five independent experiments. c Frequency of CD34⁺CD38^lo/− HSPCs within human graft found in the BM of 1^ry recipients. Mice with sufficient human graft (above the dotted line) were used for analysis in (d); median lines are shown (n = 7–12 mice). d Frequency of the denoted output sub-populations within CD34⁺CD38^lo/− HSPCs found in mice engrafted at 5 and 12 wks with the indicated cell populations (n = 6–9 mice). Percentages were calculated based on the gates shown in Supplementary Fig. 1f. n.d not detected. Bars are the mean values and error bars are the S.D for the no. of mice analyzed. Source data are provided as a Source Data file.