Fig. 3. In vivo studies support EPCR as a marker for the repopulating cells within both CD90⁺CD49f⁺ and CD90^-CD49f⁺ populations.

a Representative phenotypic analysis of EPCR⁺ cells in cord blood CD34⁺ cells. Frequencies were calculated based on the gates shown (n = 8 mice) Of note, three different commercial anti-EPCR antibodies gave very similar staining profiles (Supplementary Fig. 2c). b Frequency of EPCR⁺ cells within CD90⁺CD49f⁺, CD90⁻CD49f⁺, CD90⁺CD49f⁻ and CD90⁻CD49f⁻ populations respectively (n = 8 mice). c Engraftment of EPCR⁺ and EPCR⁻ cells within CD90⁺CD49f⁺ and CD90⁻CD49f⁺ populations (flow-sorted as shown in (b)) assayed in NSG mice 12 wks post-transplant with the denoted cell dose. Results were from three independent experiments (n = 7–12 mice); median lines are shown. d SRC frequency within (CD34⁺CD38⁻CD45RA⁻) CD90⁺CD49f⁺EPCR⁺ and CD90⁻CD49f⁺EPCR⁺ populations; 95% confidence interval is shown. ± shown is the S.D for the no. of experiments performed. The anti-CD201 clone RCR-227 was used in all these experiments. Source data are provided as a Source Data file.