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. 2022 Apr 19;12:6445. doi: 10.1038/s41598-022-09497-y

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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USP32 expression level in glioblastoma (GBM) tissues and cell lines and the association of USP32 expression level with prognosis. (A,B) USP32 is upregulated in GBM patients (A), which indicates poor prognosis (B). The USP32 mRNA expression data and survival information of GBM patients were downloaded from the Gene Expression Omnibus databases. (C,D) The USP32 mRNA and protein levels in GBM cells (U-118 MG, U-87 MG, A172, T198G, and U-251 MG) and normal brain cell SVG p12 were determined using RT-qPCR (C) and western blotting (D), respectively. Data are represented as mean ± SD of three technical replicates. Unpaired Student's t test for (A). Kaplan–Meier method and the significance was determined using the log-rank test for (B). One way ANOVA followed by Tukey’s post-hoc test for (C,D): vs SVG p12, *p < 0.05, ***p < 0.001, ****p < 0.0001.