Fig. 8. MFG-E8 upregulates Nrf2 activation through the PI3K/AKT signaling pathway in rat NP cells.

A–C Western blot and its quantification revealed the protein expressions of p-PI3K, PI3K, p-Akt, and Akt in NP cells after being treated with or without the administration of MFG-E8 with H₂O₂. D–F We evaluated the level of Nrf2 in the nucleus and the level of p-AKT and AKT in the whole cells by western blot after in after NP cells after treatment with H₂O₂ or MFG-E8 or LY294002. G The Nrf2 was detected by immunofluorescence combined with DAPI staining for nuclei. All data are presented as mean ± standard deviation (SD) of, n = 6; *p < 0.05 vs. untreated group, ^##p < 0.01 vs. H₂O₂ group, ^&&p < 0.01 vs. H₂O₂ + MFG-E8 group.