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. 2022 Apr 19;13:2070. doi: 10.1038/s41467-022-29684-9

Fig. 2. Genetic knock-down screen identifies USP25 as an essential DUB in PDAC organoids.

Fig. 2

a Schematic of genetic knock-down screen in KPCY organoids, created with BioRender.com. b Heatmap of percentage whole well growth confluency (% confluency) as measured by a high-content imaging system, 78 h post-puromycin selection. Biological replicates are shown for each individual shRNA target. c Organoid growth shown as % confluency, displayed as mean ± standard deviation (SD). Individual shRNA (black circles) values are shown for each gene, averaged from three biological replicates. Statistical significance was determined by One-way ANOVA with Dunnett post-hoc test (n = 3 biologically independent samples). d Organoid viability displayed as % relative to control shYFPs (red bars), displayed as mean ± SD. Individual shRNAs (black circles) are shown for each gene, averaged from three biological replicates. Statistical significance was determined by One-way ANOVA with Dunnett post-hoc test (n = 3 biologically independent samples). e Heatmap of mRNA expression by qPCR for each individual shRNA per gene, relative to the average of three independent shYFPs. Statistical significance was determined by two-tailed Student’s t test with Holm–Sidak post-hoc correction for multiple testing (n = 3 biologically independent samples). Source data are provided as a Source Date file.