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. 2022 Apr 19;13(4):374. doi: 10.1038/s41419-022-04826-5

Fig. 3. Combination of NOB and ROR ectopic expression in MDA-MB-231 cells suppresses cell growth and motility.

Fig. 3

A WST-1 assays. Mock indicates P3Xflag-CMV10-Neor (Empty vector). Data represent mean ± SEM. Two-way ANOVA with Sidak’s multiple comparisons test showed significant difference. ***p < 0.001. B Wound healing assays showed retarded motility in RORA- or RORC-expressing MDA-MB-231 cells. The results were expressed as the percentage of the motility of the control cells (100%, upper panel) (×100 magnification, scale bar = 277.3 μm). Data represent mean ± SEM. Two-way ANOVA with Sidak’s multiple comparisons, *p < 0.05 and ***p < 0.001. C NOB and RORA/C expression in MDA-MB-231 showed a combination effect in colony formation assays. Right panel: quantification. Data represent mean ± SEM. Two-way ANOVA with Sidak’s multiple comparisons test, **p < 0.01 and ***p < 0.001. Interaction between RORA/C expression and NOB, p = 0.0026 via two-way ANOVA.