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. 2022 Apr 19;13(4):374. doi: 10.1038/s41419-022-04826-5

Fig. 6. Combination studies show the efficacy of NOB with DTX or CAR in TNBC cells.

Fig. 6

A MDA-MB-231 cells were treated with the NOB and/or DTX for 24, 48, and 72 h and cell proliferation was determined by WST-1 assays. Data represent mean ± SEM. One-way ANOVA with Tukey’s post hoc test showed significant difference compared to DMSO, ***p < 0.001, compared to DTX, #p < 0.05, ##p < 0.01, and ###p < 0.001. Interaction, p < 0.01 via two-way ANOVA. B MDA-MB-231 cells were treated with NOB and/or CAR for 24, 48, and 72 h and cell proliferation was determined by WST-1 assay. Data represent mean ± SEM. Two-tailed Student’s t-test showed significant difference compared to DMSO, ***p < 0.001, compared to CAR 10 µM, #p < 0.05, ##p < 0.01, and ###p < 0.001. C MDA-MB-468 cells were treated with NOB (100 µM) and/or DTX (5 nM) for 48 h and cell death was determined by FACS analysis. Left panel; Representative images of flow cytometric analysis; right panel; quantification. Data represent mean ± SEM. Two-way ANOVA with Tukey’s multiple comparisons test showed significant difference compared to DMSO, ***p < 0.001, NOB, ###p < 0.001, and compared to DTX, †††p < 0.001 (interaction, p < 0.001 via two-way ANOVA). Student t-test compared to DMSO, §p < 0.05, §§p < 0.01. D DB7 cells were treated with NOB (20, 40, and 80 µM) and/or DTX (2.5 nM) for 24 and 72 h and cell proliferation was determined by WST-1 assays. Data represent mean ± SEM. Two-way ANOVA with Tukey’s multiple comparisons test showed significant difference compared to DMSO, **p < 0.01; ****p < 0.0001.