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. 2022 Apr 19;13(4):375. doi: 10.1038/s41419-022-04823-8

Fig. 5. Flubendazole-induced mitophagy enhances anti-migration potential in MDA-MB-231 cells.

Fig. 5

MDA-MB-231 cells were transfected with negative-control or DRP1 shRNA for 24 h and treatment with or without flubendazole (0.5 μM) for 24 h. A, B The scratch assay was used to measure the migration capabilities of the cells. Representative images and statistics were shown. Scale bar, 100 µm. C, D Transwell assay was used to measure the number of migrated cells. Representative images and statistics were shown Scale bar, 50 µm. E Immunoblotting of E-cadherin and MMP-2 expression. β-actin was measured as the loading control. F–I The expression of MMP-2 and E-cadherin were analyzed by immunofluorescence. Scale bar, 20 µm. Data represent mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001. Statistical significance compared with respective control groups (all P-values were obtained by one-way ANOVA).