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. 2022 Apr 19;13(4):375. doi: 10.1038/s41419-022-04823-8

Fig. 8. Silencing DRP1 partially blocks the anti-proliferative and anti-migration effects of EVA1A overexpression in breast cancer.

Fig. 8

MDA-MB-231 and MCF-7 cells were co-transfected with DRP1 shRNA and Flag-EVA1A or vehicle control respectively for 48 h. A Cell viability was measured by MTT assay. B, C Representative images and quantification of Edu-positive cells were shown. Scale bar, 50 µm. D, E Representative images and quantification of colonies were shown. F, G The scratch assay was used to measure the migration capabilities of the cells. Representative images and statistics were shown. Scale bar, 100 µm. H, I Transwell assay was used to measure the number of migrated cells. Representative images and statistics were shown. Scale bar, 50 µm. J Immunoblotting of E-cadherin and MMP-2 expression. β-actin was measured as the loading control. Data represent mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001. Statistical significance compared with respective control groups (all P-values were obtained by one-way ANOVA).