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. 2022 Apr 19;13:2080. doi: 10.1038/s41467-022-29746-y

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — a Violin plots were generated using a combined pool of cells from WT and Foxf1^WT/S52F E18.5 lungs and show endothelial-enriched expression of ETS transcription factors Fli1, Erg and Ets1. ETS transcription factor Spi1 is not expressed in endothelial, epithelial, and mesenchymal cell lineages in the lung tissue. b Violin plots show that Fli1 is enriched in gCAPs, whereas Erg is enriched in aCAPs. Ets1 expression is similar in aCAPs and gCAPs. The data for box plots were from single cell normalized counts (WT: n = 532; Foxf1^WT/S52F: n = 320). Boxplots: center line, median; box boundary, first and third quartiles; whiskers denote 5th–95th percentile. T-test (two-tailed) were performed, pvalue for Fli1 is 0.01677, and for Erg is 0.02791. c Dual luciferase (LUC) assay shows transcriptional synergy between CMV-FOXF1 and CMV-FLI1 expression vectors to activate the ACE400 LUC reporter. Co-transfection experiments were performed using fetal lung MFLM-91U cells (n = 4). Data were shown as mean ± SD, and one-way ANOVA followed by Tukey’s test (two-tailed) were performed. **p < 0.01, *p < 0.05, ns is not significant. d ChIPseq shows that FOXF1 and FLI1 proteins bind to the ACE400 region of Acvrl1 gene (red box).