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. 2022 Apr 19;13(4):373. doi: 10.1038/s41419-022-04827-4

Fig. 7. xCT activates the AKT/mTOR signaling pathway and enhances MELK expression in CRC cells.

Fig. 7

A DEGs in HCT15 cells with or without xCT shRNA administration. B KEGG analysis was performed on the DEGs. C GSEA showed that xCT was upregulated in samples with the activation of the hallmark_mTORC1_signaling pathway. D Identification of potential factors whose expression might correlate with that of xCT in CRC. E, F The correlation between the expression of MELK and xCT in GSE39582 and GSE87211. G, H IF assays confirmed that MELK and xCT were strongly colocalized in HCT116 and HCT15 cells. I, J Western blot analysis showed that xCT knockdown inhibited the expression level of MELK and restrained the activation of the AKT/mTOR signaling pathway in HCT116 and HCT15 cells.