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. 2022 Apr 19;13(4):373. doi: 10.1038/s41419-022-04827-4

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 8 — A, B The colony formation assay showed that the suppressive effect of xCT inhibition on the proliferation of CRC cells was reversed by MELK upregulation. C, D Transwell assays indicated that MELK overexpression effectively recovered the migration capability inhibited by xCT knockdown in CRC cells. E, F Cell cycle assay confirmed that MELK rescued the suppression of xCT inhibition on G1/S transition. G, H The sphere formation assay indicated that upregulated MELK rescued the suppressive effect of xCT inhibition on the sphere-forming capability of CRC cells. I The interaction of MELK with different factors expressed in various organelles. J, K GSEA indicated that upregulated expression of MELK was closely related to the activation of signaling pathways, including hallmark_mTORC1_signaling and hallmark_MYC_targets_V1. L, M Western blot analysis confirmed that upregulated MELK rescued the suppression of the expression of tumorigenesis- and stemness-related factors in CRC cells with xCT knockdown. N, O The suppressive effect of xCT knockdown on the AKT/mTOR signaling pathway was rescued by MELK overexpression. *P < 0.05, **P < 0.01, ***P < 0.001, ^# P < 0.05, ^## P < 0.01.