. 2021 Mar 8;30(21):5303–5317. doi: 10.1111/mec.15845

TABLE 1.

Non‐synonymous nucleotide variation in the voltage‐gated sodium channel gene

Variant				Population allele frequency (%)
Position ^a	Ag ^b	Md ^c	Domain ^d	AOAc	GHAc	BFAc	CIAc	GNAc	GW	GM	CMAg	GHAg	BFAg	GNAg	GAAg	UGAg	GQAg	FRAg	KE
2,390,177 G > A	R254K	R261	IL45	0.0	0.009	0.0	0.0	0.0	0.0	0.0	0.313	0.0	0.0	0.0	0.203	0.0	0.0	0.0	0.0
2,391,228 G > C	V402L	V410	IS6	0.0	0.127	0.073	0.085	0.125	0.0	0.0	0.0	0.0	0.0	0.0	0.0	0.0	0.0	0.0	0.0
2,391,228 G > T	V402L	V410	IS6	0.0	0.045	0.06	0.0	0.0	0.0	0.0	0.0	0.0	0.0	0.0	0.0	0.0	0.0	0.0	0.0
2,399,997 G > C	D466H	‐	LI/II	0.0	0.0	0.0	0.0	0.0	0.0	0.0	0.069	0.0	0.0	0.0	0.0	0.0	0.0	0.0	0.0
2,400,071 G > A	M490I	M508	LI/II	0.0	0.0	0.0	0.0	0.0	0.0	0.031	0.0	0.0	0.0	0.0	0.0	0.0	0.0	0.0	0.188
2,400,071 G > T	M490I	M508	LI/II	0.0	0.0	0.0	0.0	0.0	0.0	0.0	0.003	0.0	0.0	0.0	0.0	0.0	0.0	0.0	0.0
2,402,466 G > T	G531V	G549	LI/II	0.0	0.0	0.0	0.0	0.0	0.0	0.0	0.0	0.0	0.0	0.0	0.007	0.0	0.056	0.0	0.0
2,407,967 A > C	Q697P	Q724	LI/II	0.0	0.0	0.0	0.0	0.0	0.0	0.0	0.0	0.0	0.0	0.0	0.0	0.0	0.056	0.0	0.0
2,416,980 C > T	T791M	T810	IIS1	0.0	0.009	0.02	0.0	0.0	0.0	0.0	0.0	0.292	0.147	0.112	0.0	0.0	0.0	0.0	0.0
2,422,651 T > C	L995S	L1014	IIS6	0.0	0.0	0.0	0.0	0.0	0.0	0.0	0.157	0.0	0.0	0.0	0.674	1.0	0.0	0.0	0.76
2,422,652 A > T	L995F	L1014	IIS6	0.84	0.818	0.853	0.915	0.875	0.0	0.0	0.525	1.0	1.0	1.0	0.326	0.0	0.0	0.0	0.0
2,429,556 G > A	V1507I	‐	IIIL56	0.0	0.0	0.0	0.0	0.125	0.0	0.0	0.0	0.0	0.0	0.0	0.0	0.0	0.0	0.0	0.0
2,429,617 T > C	I1527T	I1532	IIIS6	0.0	0.173	0.133	0.085	0.125	0.0	0.0	0.0	0.0	0.0	0.0	0.0	0.0	0.0	0.0	0.0
2,429,745 A > T	N1570Y	N1575	LIII/IV	0.0	0.0	0.267	0.0	0.0	0.0	0.0	0.057	0.167	0.207	0.088	0.0	0.0	0.0	0.0	0.0
2,429,897 A > G	E1597G	E1602	LIII/IV	0.0	0.0	0.0	0.0	0.0	0.0	0.0	0.0	0.0	0.065	0.062	0.0	0.0	0.0	0.0	0.0
2,429,915 A > C	K1603T	K1608	IVS1	0.0	0.055	0.047	0.0	0.0	0.0	0.0	0.0	0.0	0.0	0.0	0.0	0.0	0.0	0.0	0.0
2,430,424 G > T	A1746S	A1751	IVS5	0.0	0.0	0.0	0.0	0.0	0.0	0.0	0.0	0.292	0.141	0.1	0.0	0.0	0.0	0.0	0.0
2,430,817 G > A	V1853I	V1858	COOH	0.0	0.0	0.0	0.0	0.0	0.0	0.0	0.0	0.542	0.049	0.062	0.0	0.0	0.0	0.0	0.0
2,430,863 T > C	I1868T	I1873	COOH	0.0	0.0	0.0	0.0	0.0	0.0	0.0	0.0	0.0	0.261	0.2	0.0	0.0	0.0	0.0	0.0
2,430,880 C > T	P1874S	P1879	COOH	0.0	0.027	0.207	0.345	0.0	0.0	0.0	0.0	0.0	0.0	0.0	0.0	0.0	0.0	0.0	0.0
2,430,881 C > T	P1874L	P1879	COOH	0.0	0.0	0.073	0.007	0.25	0.0	0.0	0.0	0.0	0.234	0.475	0.0	0.0	0.0	0.0	0.0
2,431,061 C > T	A1934V	A1939	COOH	0.0	0.018	0.107	0.465	0.5	0.0	0.0	0.0	0.0	0.0	0.0	0.0	0.0	0.0	0.0	0.0
2,431,079 T > C	I1940T	I1945	COOH	0.0	0.118	0.04	0.0	0.0	0.0	0.0	0.067	0.0	0.0	0.0	0.0	0.0	0.0	0.0	0.0

AO, Angola; GH, Ghana; BF, Burkina Faso; CI, Côte d'Ivoire; GN, Guinea; GW, Guinea‐Bissau; GM, Gambia; CM, Cameroon; GA, Gabon; UG, Uganda; GQ, Bioko; FR, Mayotte; KE, Kenya; Ac, An. coluzzii; Ag, An. gambiae. Species status of specimens from Guinea‐Bissau, Gambia and Kenya is uncertain (The Anopheles gambiae 1000 Genomes Consortium, 2020). All variants are at 5% frequency or above in one or more of the 16 Ag1000G phase 2 populations, with the exception of 2,400,071 G > T which is only found in the CMAg population at 0.3% frequency but is included because another mutation is found at the same position (2,400,071 G > A) at >5% frequency and which causes the same amino acid substitution (M490I).

^{^a}

Position relative to the AgamP3 reference sequence, chromosome arm 2L.

^{^b}

Codon numbering according to Anopheles gambiae transcript AGAP004707‐RD in geneset AgamP4.12.

^{^c}

Codon numbering according to Musca domestica EMBL accession X96668 (Williamson et al., 1996).

^{^d}

Location of the variant within the protein structure. Transmembrane segments are named according to domain number (in Roman numerals) followed by ‘S’ then the number of the segment; for example, ‘IIS6’ means domain two, transmembrane segment six. Internal linkers between segments within the same domain are named according to domain (in Roman numerals) followed by ‘L’ then the numbers of the linked segments; for example, ‘IL45’ means domain one, linker between transmembrane segments four and five. Internal linkers between domains are named ‘L’ followed by the linked domains; for example, ‘LI/II’ means the linker between domains one and two. ‘COOH’ means the internal carboxyl tail.