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. 2022 Mar 18;298(5):101844. doi: 10.1016/j.jbc.2022.101844

Figure 10.

Figure 10

Schematic representation of the dissociation of PABPC1 from poly(A) by Paip2A. A, in the poly(A)-bound PABPC1, the RRM2–RRM3 region mainly contributes to the poly(A) association. B, as the affinity of the RRM2 region for poly(A) (Kd = 200 μM, Table 1) is 50-fold weaker than that for Paip2A (Kd = 4.0 μM, Table 2), the RRM2 region transfers from poly(A) to RRM2-binding region of Paip2A (blue) when Paip2A approaches sites where the RRM3-binding region of Paip2A (yellow) are partly available for RRM3 binding. Next, Paip2A removes RRM3 from poly(A) because of the threefold higher affinity of RRM3 for Paip2A (Kd = 1.3 μM, Table 2) compared with that for poly(A) (Kd = 4.7 μM, Table 1). PABPC1, poly(A)-binding protein C1; Paip2A, PABP-interacting protein 2A; RRM, RNA recognition motif.