Table 2.
Thermodynamic parameters obtained via ITC analysis of the interaction of RRM or RRM2/3 with Paip2A(25–83)
| RRM | Na | Kd (M) | ΔH (kJ/mol) | ΔG (kJ/mol) | ΔS (kJ mol−1 K−1) |
|---|---|---|---|---|---|
| RRM1 | ND | ND | ND | ND | ND |
| RRM2 | 0.87 ± 0.03 | (4.0 ± 0.5) × 10−6 | −1.2 × 102 | −31 | −0.31 |
| RRM3 | 0.87 | (1.3 ± 0.1) × 10−6 | −1.8 × 102 | −34 | −0.50 |
| RRM4 | ND | ND | ND | ND | ND |
| RRM2/3 | 0.83 | (1.9 ± 0.6) × 10−9 | −2.3 × 102 | −50 | −0.61 |
Abbreviation: ND, not detected.
Paip2A(25–83): The peptide with residues 25 to 83 of PABP-interacting protein 2A.
For N, Kd, and ΔH, if the value of error is less than significant, the error is not stated.
a
N is the molar ratio of RRM to Paip2A(25–83).