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. 2022 Mar 5;4(5):100463. doi: 10.1016/j.jhepr.2022.100463

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© 2022 The Authors

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

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Fig. 1 — Generation of Ostβ^-/- mice.

(A) Schematic representation of the wild-type OSTβ and knockout OSTβ gene resulting from CRISPR/Cas9-mediated deletion of exon 3. (B) OSTα and OSTβ mRNA expression in ileums of 4- and 8-week-old male wild-type, Ostα^-/- and Ostβ^-/- mice. Data are normalized using the geometric mean of CyclophillinB and Rpl4 (n = 5–7 mice per group). Statistical analysis was done using a one-way ANOVA test and Dunnett’s test to compare with wild-type littermates. ∗Indicates p value of <0.05. (C) OSTα and OSTβ protein expression in ileums of 4-week-old female wild-type, Ostα^-/- and Ostβ^-/- mice. Na/K-ATPase is used as loading control. (D) Immunohistochemistry on ileal sections from wild-type and Ostβ^-/- mice stained with antibody against OSTβ. Original magnification, 400x. Scale bar 25 μm. bp, base pair; del, deletion; KO, knockout; Ostα, organic solute transporter alpha; Ostβ, organic solute transporter beta; WT, wild-type.