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. 2022 Mar 5;4(5):100463. doi: 10.1016/j.jhepr.2022.100463

Fig. 3.

Fig. 3

The phenotype of Ostα-/- and Ostβ-/- mice compared with wild-type littermates at 4 and 8 weeks of age in females and males.

(A) Representative microscopic pictures of H&E-stained transverse sections of the distal ileum (n = 4-8 per group) in male mice. Original magnification, 100x. Scale bar 100 μm. (B-E) Quantitative analysis of ileal sections measured from 5 field views per mouse (n = 4-8). (B) Villus height presented as mean ±SD with individual points showing the mean villus height per mouse (30-90 villi) (C) Crypt depth presented as mean ±SD with individual points showing the mean crypt depth per mouse (30-90 villi). (D) Representative microscopic picture of Alcian Blue staining on paraffin-embedded ileal sections from Ostα-/-, Ostβ-/- and wild-type 8-week-old female mice (n = 3). Original magnification, 200x. Scale bar 50 μm. (E) Representative pictures of phosphohistone H3 staining on ileal sections from Ostα-/-, Ostβ-/- and wild-type 8-week-old female mice (n = 3). Original magnification, 100x. Scale bar 100 μm. Statistical analysis was performed using a one-way ANOVA test, and Dunnett’s test to compare with wild-type littermates. ∗Indicates p values of <0.05. H&E, hematoxylin and eosin; Ostα, organic solute transporter alpha; Ostβ, organic solute transporter beta; wk, week; WT, wild-type.