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RT-PCR analysis of HEp-2 cells treated with ofloxacin. Cells were infected at an MOI of 0.05 and incubated with 2 μg of ofloxacin per ml starting 2 days postinfection. RNA from cell pellets was prepared and analyzed by RT-PCR as described in Materials and Methods. Lanes: 1, marker; 2, uninfected cells; 3, control cells 2 days postinfection; 4 to 7, chlamydia-infected cells treated with ofloxacin on the indicated day (d).
