FIGURE 3.

Expression and apical localization of OAT10 in polarized MDCKII cells. Immunoblot detection of OAT10 and URAT1 in whole cell lysate samples prepared from MDCKII cells 72 h after transfection (A). OAT10 or URAT1 fused with EGFP was detected using an anti-EGFP antibody. PNGase F sensitive signals indicate that the target proteins were maturated as glycoproteins; α-tubulin, loading control. Representative confocal microscopic Z-sectioning images (B). An endogenous apical membrane marker gp135 was immunostained using an anti-gp135 antibody (red). Nuclei were stained with TO-PRO-3 iodide (gray). Mock (EGFP), a control vector.