Figure 2.

Efficient indel generation of oca2-coding sequence by CRISPR/Cas9. (a) Four sgRNAs were designed to cut the genomic sequence at exon 1 and exon 3. Two injection mixes were used, one containing sgRNA1 and sgRNA2 targeting exon 1 and exon 3, and the other containing sgRNA3 + sgRNA4 both targeting exon 1. Alignment of mutant F1 individuals derived from crosses #1, #3 and #4 are shown for (b) sgRNA1, (c) sgRNA2 and (d) sgRNA4. All F1 individuals were wild-type at the cutting site of sgRNA3.