Figure 13.

N-acetylcystein (NAC) prevents only 2-methoxyestradiol (2ME)–induced apoptosis but not TRAIL-mediated apoptosis. Cell viability was measured by the MTS assay, and the data represented as a percentage in respect to control (vehicle, C). Standard deviation of the mean is shown from a minimum of 3 experiments performed, each consisting of 5 replicates. The antioxidant NAC was coincubated at 10 μM with 2ME (5 μM) for 48 hours and TRAIL (200 ng/mL) for 18 hours. Using the Mann-Whitney test, significance was set at P < .05, with a = significant in respect to vehicle, b = significant in respect to 2ME (5 uM) in A2780 cells.