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. Author manuscript; available in PMC: 2022 Apr 20.
Published in final edited form as: Reprod Sci. 2008 Nov;15(9):878–894. doi: 10.1177/1933719108324171

Figure 15.

Figure 15.

Bcl-xL overexpression does not affect Bid cleavage (tBid) upon 2-methoxyestradiol (2ME) or 2ME plus TRAIL treatment and abrogates cytochrome c and apoptosis inducing factor (AIF) release induced by 2ME. A, Western blot analysis of Bcl-xL and tBid after treatment with ethanol vehicle (Control), TRAIL 200 ng/mL for 18 hours, 2ME for 48 hours or 2ME for 48 hours with TRAIL addition occurring 18 hours before the end of the experiment (2ME + TRAIL) in the A2780/wt (vector) and A2780/Bcl-xL cell lines. B, Western blot analysis of intrinsic apoptotic proteins, cytochrome c and AIF between the cytoplasm (cytosol) and the mitochondrial membrane (mitochondria) at varying time points after 2ME (5 μM) addition (hours) in the A2780/Bcl-xL cell line.