2-Methoxyestradiol (2ME) mediates activation of the intrinsic and extrinsic apoptotic pathways in A2780 cells. Western blot analysis of intrinsic and extrinsic apoptotic pathway proteins at varying time points after 2ME (5 μM) addition (hours). Western blot analysis of cytosolic and membrane factions was performed to examine the translocation of (A) BAD, BAX Cytochrome c, SMAC/Diablo, Bcl-2, Bcl-xL, and FLIP and (B) apoptosis inducing factor (AIF) apoptotic protein between the cytoplasm (cytosol), mitochondrial membrane (mitochondria), and nucleus.