Skip to main content
. Author manuscript; available in PMC: 2022 Apr 20.
Published in final edited form as: Reprod Sci. 2008 Nov;15(9):878–894. doi: 10.1177/1933719108324171

Figure 8.

Figure 8.

Downregulation of superoxide dismutase type 1 by 2-methoxyestradiol (2ME). A, RT-PCR analysis of superoxide dismutase (SOD) type I mRNA in A2780 cells in response to varying concentrations of 2ME for 9 hours. GAPDH is shown as a loading control. B, Cell viability was measured by the MTS assay, and the data represented as a percentage in respect to control (vehicle, C). Standard deviation of the mean is shown from a minimum of 3 experiments performed, each consisting of 5 replicates. The antioxidant N-acetylcystein (NAC) was coincubated at 0.1, 1, 5, and 10 mM with 2ME (5 μM) for 48 hours. Using the Mann-Whitney test, significance was set at P < .05, with ★ = significant in respect to vehicle, # = significant in respect to 2ME (5 μM).