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. 2022 Apr 6;13:869825. doi: 10.3389/fimmu.2022.869825

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Copyright © 2022 Sun, Li, Mellors, Orentas and Dimitrov

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Characterization of constructed library by bio-panning against TGFb1 and MSLN. (A) ELISA of binders against TGFb1; (B) ELISA of binders against MSLN; (C) Dynamic light scattering analysis for evaluation of the aggregation propensity of the TGFb1 specific VH domains; 3 different colors represent 3 different repeats; (D) Dynamic light scattering analysis for evaluation of the aggregation propensity of the MSLN specific VH domains, 3 different colors represent 3 different repeats.