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. 2022 Apr 6;13:869825. doi: 10.3389/fimmu.2022.869825

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Copyright © 2022 Sun, Li, Mellors, Orentas and Dimitrov

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Characterization of VH domains 1-16 and 1-16-3. (A) Flow cytometry analysis for binding of VH 1-16 and 1-16-3 to PD-L1 expressed on the surface of CHOK1 cells; blue peaks represent cells stained with AF488 conjugated secondary antibody only, red peaks represent cells stained with isotype control and then AF488 conjugated secondary antibody, yellow peaks represent cells stained with VH domains and then AF488 conjugated secondary antibody; (B) Dynamic light scattering analysis for evaluation of the aggregation propensity of domain s domains 1-16 and 1-16-3; Curves with different color are different repeats; (C) ELISA of 1-16 and 1-16-3 against PD-L1 with or without human IgG1 Fc, and their competition; (D) Structural modelling of PD-L1 with VH 1-16. Epitopes were labeled in the figure.