Table 1.

Primers for amplification of natural occurring full length VH gene repertoires.

Forward primers fornaturally occurring human VH fragments	Sequences (5’-3’)	Antibody VH sub-families amplified
VH-F1	CAG RTG CAG CTG GTG CAR TCT GG	IGHV 1
VH-F2	SAG GTC CAG CTG GTR CAG TCT GG	IGHV 1
VH-F3	CAG RTC ACC TTG AAG GAG TCT GG	IGHV 2
VH-F4	SAG GTG CAG CTG GTG GAG TCT GG	IGHV 3
VH-F5	GAG GTG CAG CTG GTG GAG WCY GG	IGHV 3
VH-F6	CAG GTG CAG CTA CAG CAG TGG GG	IGHV 4
VH-F7	CAG STG CAG CTG CAG GAG TCS GG	IGHV 4
VH-F8	GAR GTG CAG CTG GTG CAG TCT GG	IGHV 5
VH-F9	CAG GTA CAG CTG CAG CAG TCA GG	IGHV 6
Reverse primers for naturally occurring human VH fragments	Sequences (5’-3’)	Antibody VH sub-families amplified
VH-R1	CTG GTC ACY GTC TCC TCA	IGHJ1, IHGJ2, IGHJ4, IGHJ5
VH-R3	ATG GTC ACC GTC TCT TCA	IGHJ3
VH-R6	ACG GTC ACC GTC TCC TCA	IGHJ6