Figure 3.

The whole process of NT-ETR

IVF-derived BDF1 mouse embryos are injected with or without LacR-mCherry mRNA solution at 6 hpi and cultured in KSOMaa medium containing 0.1 μg/mL demecolcine from 40 hpi. At 46 hpi, NT of differentiated donor cells is performed. The NT embryos are further cultured for 2 or 24 h and subjected to immunofluorescence staining, qRT-PCR or RNA-seq analyses. IVF represents in vitro fertilization. Figure 3 is modified from the original Figure 3 of Tomikawa et al. (2021).