Skip to main content
. 2022 Apr 11;3(2):101284. doi: 10.1016/j.xpro.2022.101284

Figure 4.

Figure 4

Technical points during NT-ETR procedure

(A) Drops of reagents for NT-ETR, set on the 10 cm dish lid.

(B) Our manipulation system for NT-ETR. Holding and injection pipettes are set on the manipulator, equipped with the XYClone laser system.

(C) Aspiration of two donor cells together with HVJ-E solution just before injection of the cells to a 4-cell embryo. Two to three times more volume of HVJ-E solution than donor cells is needed for successful fusion of donor cells with a 4-cell embryo.

(D) The process of making a hole in the zona pellucida of a recipient 4-cell embryo by the XYClone laser system (I and II). Red pointer in the black circle marks the place for laser illumination. Scale bar, 20 μm.

(E) Injection of two donor cells and HVJ-E solution into the perivitelline space of a recipient 4-cell embryo through the hole of zona pellucida, produced by XYClone laser illumination (I–IV). Scale bar, 20 μm.