ES cell differentiation medium

Reagent	Final concentration	Amount
G-MEM with L-Glutamine and Phenol Red	n/a	155.88 mL
Fetal Bovine serum (FBS)	20%	40 mL
Sodium pyruvate (100 mM)	1 mM	2 mL
Non-essential amino acids (NEAA)	1/100 of the stock	2 mL
Retinoic acid (10 mM)	1 μM	20 μL
Penicillin/Streptomycin stock	20 U/mL/20 μg/mL	100 μL
Total	n/a	200 mL

Store at 4°C for 1 month.