Table 1.

Reaction numbers and sizes according to variants in the library

Variants in library/number of reactions	PCR reaction	Ligation reaction	electroporation cuvette	14 cm plate	LB medium (mL)
600–2,000	5	1	1–3	12	130
2,000–5,000	5	2	3–5	20	210
5,000–15,000	5	2	4–6	24	250
15,000–55,000	16	2	7	28	290