Reagent	Amount (μL)
Buffer (5×)	10
dNTPs (2.5 mM each)	5
Primer Forward (20 uM)	2.5
Primer Reverse (20 uM)	2.5
Herculase polymerase	1
Diluted DNA (e.g., 1:10 or 1:50)	5
DNase free double distilled water	24
Total	50