| PCR cycling conditions | |||
|---|---|---|---|
| Steps | Temperature | Time | Cycles |
| Initial Denaturation | 95°C | 1 min | 1 |
| Denaturation | 95°C | 20 s | 10–14 cycles |
| Annealing and extension | 68°C | 1 min | |
| Final extension | 68°C | 4 min | 1 |
| Hold | 4°C | Forever | 1 |
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