| Reagent | Amount (μL) |
|---|---|
| Buffer FD (10×) | 6 |
| Plasmid DNA (e.g., 2 μg) | X |
| Restriction enzyme 1 | 3 |
| Restriction enzyme 2 | 3 |
| DNase free double distilled water | 48-x |
| Total | 60 |
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