Reagent	Amount
Buffer (10×)	1
Library DNA	150/(4213/123) = 4.39 ng (3.4 μL of 1.3 ng/μL)
Plasmid DNA	150 ng (2.7 μL of 54.8 ng/μL)
Ligase Lucigen Fast Link	1
ATP	1
DNase free double distilled water	Up to 10 μL (here 0.9 μL)
Total	10 μL