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. 2022 Jan 17;3(1):101103. doi: 10.1016/j.xpro.2021.101103
PCR cycling conditions
Steps Temperature Time Cycles
Initial Denaturation 95°C 10 min 1
Denaturation 95°C 30 s 32 cycles
Annealing 56°C 30 s
Extension 72°C 1 min
Final extension 72°C 10 min 1
Hold 10°C Forever