KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Bcd rabbit antibody | Wieschaus lab | N/A |
| Hb mouse antibody | Wieschaus lab | N/A |
| GFP sheep antibody | Bio-Rad | Cat # 4745–1051; RRID:AB_619712 |
| Donkey anti sheep, Alexa-488 | Invitrogen | Cat # A-11015; RRID:AB_141362 |
| Donkey anti mouse, Alexa-594 | Invitrogen | Cat # R37115; RRID:AB_2556543 |
| Donkey anti rabbit, Alexa-647 | Invitrogen | Cat # A32795; RRID:AB_2762835 |
| Bacterial and virus strains | ||
| Stellar Chemically Competent Cells | ClonTech Laboratories | Cat # 636763 |
| Chemicals, peptides, and recombinant proteins | ||
| ClonAmp HiFi PCR polymerase | ClonTech Laboratories | Cat # 639298 |
| PrimeSTAR GXL DNA Polymerase | ClonTech Laboratories | Cat # R050B |
| inFusion HD cloning kit | ClonTech Laboratories | Cat #638911 |
| Halocarbon Oil 700 | Sigma | Cat # H8898 |
| Halocarbon Oil 27 | Sigma | Cat # H8773 |
| 37% Formaldehyde solution | Sigma | Cat # F8775 |
| Heptane | Sigma | Cat # 34873 |
| Methanol | Sigma | Cat # 34860 |
| PBS | Gibco | Cat # 14190144 |
| Hoyer’s solution | Wieschaus lab | N/A |
| Qiagen miniprep kits | Qiagen | Cat #27106 |
| NucleoSpin gel and PCR clean-up kits | ClonTech Laboratories | Cat # 740609 |
| Experimental models: Organisms/strains | ||
| bodE1 nosBN tsi4 | Wieschaus lab | N/A |
| bodE1 nosL7 tsi4 | Wieschaus lab | N/A |
| Sp, nos-tub | Wieschaus lab, Gavis lab | N/A |
| nos>φ NLS-MCP-mNeonGreen | This study | N/A |
| αTub67C> NLS-mCherry-LEXY | This study | N/A |
| αTub67C> NLS-eGFP-LEXY | This study | N/A |
| αTub67C> eGFP-Bcd-LEXY FRT bcd 3’ UTR hsp70 RFP FRT sqh 3’UTR | This study | N/A |
| αTub67C> Bcd-LEXY FRT bcd 3’ UTR hsp70 RFP FRT sqh 3’UTR | This study | N/A |
| αTub67C> iRFP-Bcd-LEXY FRT bcd 3’ UTR hsp70 RFP FRT sqh 3’UTR | This study | N/A |
| αTub67C> mCherry-Bcd-LEXY FRT bcd 3’ UTR hsp70 RFP FRT sqh 3’UTR | This study | N/A |
| HisGFP | Bloomington | Cat # 5941 |
| CyO; TM3, sb | Bloomington | Cat # 59967 |
| hb BAC > MS2 | (Bothma et al., 2015) | N/A |
| kni BAC > MS2 | (Bothma et al., 2015) | N/A |
| kni ΔDistal > MS2 | (Bothma et al., 2015) | N/A |
| kni ΔProx> MS2 | (Bothma et al., 2015) | N/A |
| Kr (CD1+CD2) > MS2 | (El-Sherif and Levine, 2016) | N/A |
| gt > MS2 | (Syed etal., 2021) | N/A |
| Recombinant DNA | ||
| αTub67C> eGFP-Bcd FRT bcd 3’ UTR hsp70 RFP FRT sqh 3’UTR | (Hannon et al., 2017) | N/A |
| αTub67C> Bcd-LEXY FRT bcd 3’ UTR hsp70 RFP FRT sqh 3’UTR | This paper | Addgene #182594 |
| αTub67C> iRFP-Bcd-LEXY FRT bcd 3’ UTR hsp70 RFP FRT sqh 3’UTR | This paper | Addgene #182595 |
| αTub67C> mCherry-Bcd-LEXY FRT bcd 3’ UTR hsp70 RFP FRT sqh 3’UTR | This paper | Addgene #182596 |
| αTub67C> eGFP-Bcd-LEXY FRT bcd 3’ UTR hsp70 RFP FRT sqh 3’UTR | This paper | N/A |
| αTub67C> NLS-mCherry-LEXY | This paper | Addgene #182597 |
| αTub67C> NLS-eGFP-LEXY | This paper | N/A |
| Software and algorithms | ||
| MATLAB R2021a | MathWorks | RRID: SCR_001622 |
| Python 3.10 | Python Programming Language | RRID:SCR 008394 |
| Fiji | (Schindelin et al., 2012) | http://fiji.sc; RRID: SCR_00228 |
| National Instruments/Labview | National Instruments Corp. | RRID:SCR_014325 |
| DLP 4500 LightCrafter control software | Texas Instruments | N/A |
| custom code | Zenodo&Github | https://doi.org/10.5281/zenodo.6037829 |
| Other | ||
| DLP 4500 LightCrafter unit | Texas Instruments | Cat # 296–36420-ND |