Table 4.
The orthogonal experiment results of strain D-1 protoplast preparation.
| S. no | A | B | C | D | |
|---|---|---|---|---|---|
| NaCl (M) | Enzymes | Time (h) | Temperature (°C) | Protoplast yield (106 cells·ml−1) | |
| 1 | 0.65 | 20 mg/ml of Lys + 20 mg/ml of Dri | 4 | 26 | 1.575 ± 0.197 |
| 2 | 0.65 | 15 mg/ml of Lys + 15 mg/ml of Dri | 5 | 28 | 1.242 ± 0.208 |
| 3 | 0.65 | 20 mg/ml of Lys + 10 mg/ml of Dri | 6 | 30 | 1.292 ± 0.139 |
| 4 | 0.7 | 20 mg/ml of Lys + 20 mg/ml of Dri | 5 | 30 | 2.067 ± 0.331 |
| 5 | 0.7 | 15 mg/ml of Lys + 15 mg/ml of Dri | 6 | 26 | 2.000 ± 0.354 |
| 6 | 0.7 | 20 mg/ml of Lys + 10 mg/ml of Dri | 4 | 28 | 1.517 ± 0.280 |
| 7 | 0.75 | 20 mg/ml of Lys + 20 mg/ml of Dri | 6 | 28 | 6.150 ± 1.427 |
| 8 | 0.75 | 15 mg/ml of Lys + 15 mg/ml of Dri | 4 | 30 | 2.000 ± 0.235 |
| 9 | 0.75 | 20 mg/ml of Lys + 10 mg/ml of Dri | 5 | 26 | 1.792 ± 0.196 |
| K1 | 4.109 | 9.792 | 5.092 | 5.367 | |
| K2 | 5.584 | 5.242 | 5.101 | 8.909 | |
| K3 | 9.942 | 4.601 | 9.442 | 5.359 | |
| R | 5.833 | 5.191 | 4.350 | 3.550 |