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. 2022 Apr 6;13:857932. doi: 10.3389/fphar.2022.857932

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Copyright © 2022 Chen, Fan, Ma, Wang, Tian, Li and Gong.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The experimental schema and representative HPLC chromatogram of BCD. (A) POI mouse model development and treatment protocol. (B) HPLC analysis of BCD: Mobile phase was acetonitrile/ultrapure water (containing 0.1% phosphoric acid) gradient for 60 min with a flow of 1 ml/min. Peaks: 1. peoniflorin, 2. ferulic acid, 3. typhaneoside, 4. hyperoside, 5. isorhamnetin-3-O-neohesperidoside, 6. specnuezhenide, 7. cyasterone and 8. asperosaponin VI.