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. 2022 Mar 28;23(5):353. doi: 10.3892/etm.2022.11280

Figure 1.

Figure 1

Hemorrhage-induced thalamic pain decreases miR-223 expression levels and activates the NLRP3 inflammasome. Microinjection of Coll IV, rather than saline, into the ventral posterior lateral and ventral posterior medial nuclei contributed to a significant increase in paw withdrawal frequency in response to (A) 0.07 g and (B) 0.4 g von Frey filaments and a significant decrease in paw withdrawal latency to (C) thermal and (D) cold stimuli on the contralateral side of the mice. (E-G) Both saline and Coll IV were injected into the ventral posterior lateral nuclei and ventral posterior medial nuclei, which did not change the ipsilateral side behavior. n=8 mice/group. (H) miR-223 expression levels were significantly reduced at day 1, 3, 7 and 14 days in the Coll IV-treated cohort. (I-L) The NLRP3 inflammasome was activated and caspase-1, ASC and NLRP3 were significantly elevated at day 1, 3, 7 and 14 in the Coll IV-treated group. n=3 mice/group. *P<0.05 vs. saline group at the corresponding time points. miR, microRNA; NLRP3, NLR family pyrin domain containing 3; Coll IV, collagenase IV; ASC, apoptosis-associated speck-like protein containing a CARD.