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. Author manuscript; available in PMC: 2023 Dec 1.
Published in final edited form as: FEBS J. 2021 Oct 2;289(24):7631–7669. doi: 10.1111/febs.16206

Figure 4.

Figure 4.

EBV-positive diffuse large B cell lymphoma, not otherwise specified (NOS) (A-D) and plasmablastic lymphoma (E-J). (A) H&E section (400x) of this case shows variable sizes, but there is a significant number of large cells. (B) CD20 immunostain (400x) highlights diffuse proliferation of neoplastic cells. (C) These lymphomatous cells are positive for EBV-encoded small RNA (400x). (D) Ki-67 immunostain (400x) exhibits a very high proliferative index. (E) At this medium power of H&E (200x), the plasmablastic lymphoma shows a diffuse pattern with scattered tingible-body macrophages. (F) At this higher power magnification of H&E (400x), the lymphomatous cells show pleomorphism with vesicular chromatin. Some nuclei contain single prominent nucleoli (immunoblastic morphology). Mitotic figures and karyorrhectic debris are frequently present. (G) In situ hybridization (ISH) for EBV-encoded small RNA (400x) shows positivity in essentially all lymphoma cells. (H) Kappa ISH (400x) in this case demonstrates that the tumor cells express the immunoglobulin kappa light chain. (I) Lambda ISH (400x) in this same case is negative in lymphoma cells, confirming kappa light chain restriction. (J) The PBL cells typically show diffuse and strong nuclear expression of MUM1/IRF-4 (400x). In this case, CD138 (syndecan-1) is also diffusely positive (not shown) to support plasmacytic differentiation.