Table 1. Panel of Chemicals Selected for the Present Studya.
| chemical | known biological effects | CAS number | concentration tested (μM) |
|---|---|---|---|
| phenoxyethanolb | antimicrobial activity | 122-99-6 | 0.01–0.1–1–10–100–1000 |
| niacinamideb | antipruritic, antimicrobial, vasoactive, photo-protective, sebostatic | 98-92-0 | 1–4–10–100–1000–10,000 |
| coumarinb | CYP450 | 91-64-5 | 0.001–0.01–0.1–1–10–100 |
| caffeineb | CNS stimulant | 58-08-2 | 0.01–0.1–1–10–100–1000 |
| andrographolide | anti-inflammatory | 5508-58-7 | HepG2: 0.2–0.5–2–5–10–15 |
| HepaRG: 0.2–0.5–2–5–15–80 | |||
| MCF-7: 0.2–0.5–2–5–15–80 | |||
| triclosan | fatty acid synthesis inhibitor | 3380-34-5 | HepG2: 0.1–0.4–1–4–10–20 |
| HepaRG: 0.1–0.4–1–4–10–50 | |||
| MCF-7: 0.1–0.4–1–4–10–50 | |||
| flutamide | AR antagonist | 13311-84-7 | HepG2: 0.2–0.5–2–5–20–50 |
| HepaRG: 0.2–0.5–2–5–20–200 | |||
| MCF-7: 0.2–0.5–2–5–20–200 |
a
For each chemical, the tested concentrations are reported along with the chemical mode of action (MoA) and the CAS number. The differences in concentrations tested across different cell lines were due to different sensitivities assessed by the cell viability assay (see the section Cell Viability Assay for details).
b
The tested concentrations were all the same across the three cell lines.