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. Author manuscript; available in PMC: 2022 Apr 20.
Published in final edited form as: Curr Biol. 2020 Dec 3;31(3):591–600.e4. doi: 10.1016/j.cub.2020.10.098

Figure 2. Strand asymmetry in CG and CHG methylation.

Figure 2.

Methylation at the Watson (x) and Crick (y) strand is visualized using a scatter of points with kernel density estimations in two dimensions. (A) In cell cultures, CG methylation becomes transiently asymmetric during S phase, but is rapidly restored in G2, while CHG methylation remains asymmetric throughout the entire cell cycle. (B) DNA methylation in mitotic nuclei during pollen development shows a pattern of CG symmetry and CHG asymmetry in sperm cells that is consistent with their cell cycle stage (arrested in early S phase). In contrast, symmetric mCHG is retained in microspores (G2/M) and the vegetative nucleus (G1/G0). Top left numbers show methylation values with highest density of cytosines, and the bottom right numbers represent the number of filtered cytosines analyzed in each plot. See also Figure S2 and S3B.