Figure 7.

Toxic acetaminophen (APAP) exposure induces exaggerated hepatic signal transducer and activator of transcription 3 (STAT3) activation in AKBI mice. Wild type (WT) and AKBI (IkappaB beta knock-in mice) were exposed to APAP (280 mg/kg, IP) for 0 or 24 h. A, Total serum Il-6 protein. B, Representative Western blot of phosphorylated STAT3 (pSTAT3) and STAT3 in whole liver homogenates, with total protein shown as loading control. Densitometric analysis of C, pSTAT3, D, Total STAT3, and E, pSTAT3/Total STAT3 ratio. White columns represent WT mice and black columns represent AKBI mice (n = 4–9). Data are expressed as mean ± SEM; *p < .05 versus genotype-matched control and †p < .05 versus time-matched exposure.