AKT/mTOR and β‐catenin activation by UBE2T overexpression. (A) Western blot analysis for the detection of protein levels of the components of the PI3K/AKT/mTOR pathway. (B) Quantification of the ratio of proteins: pAKT(T308)/AKT, pAKT(S473)/AKT, and pmTOR/mTOR from (A). (C) Western blot analysis for the detection of protein levels of total β‐catenin. (D) Quantification of β‐catenin from (C). Data shown are the mean ± SEM from n = 4 independent experiments, two‐tailed Student's t‐test (*P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001, ****P ≤ 0.0001). Empty vector (EV)‐transfected cells were used as control for UBE2T ectopic overexpression (UBE2T), while non‐targeting siRNA (SCRAMBLE) as control for UBE2T silencing (si‐UBE2T#1, si‐UBE2T#2). Untransfected cells (UN) were used as negative control. Normalization in western blot was performed to GAPDH.