Table 2.
Strains, plasmids, and primers used in this study
| Strains/plasmids/primers | Relevant genotype or phenotype | Sources |
|---|---|---|
| Strains | ||
| E. coli JM109 | recA1, endA1, gyrA96, thi−1, hsd R17(rk − mk +), supE44 | Invitrogen |
| E.coli JM109/pMA5-DSdpe | E. coli JM109 containing pMA5-DSdpe (AmpR) | This study |
| E. coli JM109/pMA5-RCdpe | E. coli JM109 containing pMA5-RCdpe (AmpR) | This study |
| B. subtilis 168 | Wild type | Laboratory stock |
| SDS | B. subtilis 168 containing plasmid pMA5-DSdpe (KmR) | This study |
| SRC | B. subtilis 168 containing plasmid pMA5-RCdpe (KmR) | This study |
| SDS-RC | B. subtilis 168 containing plasmid pMA5-DSdpe-RCdpe (KmR) | This study |
| SRC-DS | B. subtilis 168 containing plasmid pMA5-RCdpe-DSdpe (KmR) | This study |
| SH-H | B. subtilis 168 containing plasmid pMA5-DSdpe-PHpaII-RCdpe (KmR) | This study |
| SH-P43 | B. subtilis 168 containing plasmid pMA5-DSdpe-P43-RCdpe (KmR) | This study |
| SH-spovG | B. subtilis 168 containing plasmid pMA5-DSdpe-PspovG-RCdpe (KmR) | This study |
| SH-srfA | B. subtilis 168 containing plasmid pMA5-DSdpe-PsrfA-RCdpe (KmR) | This study |
| SP43-srfA | B. subtilis 168 containing plasmid pMA5-P43-DSdpe-PsrfA-RCdpe (KmR) | This study |
| SspovG-srfA | B. subtilis 168 containing plasmid pMA5-PspovG-DSdpe-PsrfA-RCdpe (KmR) | This study |
| Plasmids | ||
| pMA5 | HpaII promoter, colE1, rep B, replicates in E. coli (AmpR) or B. subtilis (KmR) | Laboratory stock |
| pMA5-DSdpe | pMA5 derivative carrying gene DSdpe | This study |
| pMA5-RCdpe | pMA5 derivative carrying gene RCdpe | This study |
| pMA5-DSdpe-RCdpe | pMA5 derivative carrying gene DSdpe and RCdpe, sharing HpaII promoter | This study |
| pMA5-RCdpe-DSdpe | pMA5 derivative carrying gene RCdpe and DSdpe, sharing HpaII promoter | This study |
| pMA5-DSdpe-PHpaII-RCdpe | pMA5-DSdpe-RCdpe derivative carrying gene DSdpe、promoter HpaII and gene RCdpe | This study |
| pMA5-DSdpe-P43-RCdpe | pMA5-DSdpe-RCdpe derivative carrying gene DSdpe、promoter P43 and gene RCdpe | This study |
| pMA5-DSdpe-PspovG-RCdpe | pMA5-DSdpe-RCdpe derivative carrying gene DSdpe、promoter spovG and gene RCdpe | This study |
| pMA5-DSdpe-PsrfA-RCdpe | pMA5-DSdpe-RCdpe derivative carrying gene DSdpe、promoter srfA and gene RCdpe | This study |
| pMA5-P43-DSdpe-PsrfA-RCdpe | pMA5-DSdpe-PsrfA-RCdpe derivative promoter HpaII replaced by the promoter P43 | This study |
| pMA5-PspovG-DSdpe-PsrfA-RCdpe | pMA5-DSdpe-PsrfA-RCdpe derivative promoter HpaII replaced by the promoter spovG | This study |
| primers | ||
| P1 | 5'-AAGTGAAATCAGGGGATCATGAACACGGAACGGAATTACTGCCTATTG -3' | This study |
| P2 | 5’-TTTCGACCTAGAGAACGCGTTTAGGTGGTGGTGGGTGGGTGGTTTCCATCCACACATATTTTCTGGAAATGCAAC -3’ | This study |
| P3 | 5'-AAGTGAATCAGGGGGGGATGATGATAGAAACATGGTATACTACGCATTGG -3' | This study |
| P4 | 5'-TTTCGACCTCAGAAACGCGTTTAGGTGGTGGTGGGTGGGTGGGTGGGTGGGTGGTGGTGTTGATGATGATTCAATACTGGAGA -3' | This study |