Fig. 2.

Tan-I mitigates renal fibrosis in UUO mice. A Study design. Wide type C57 mice received sham or UUO operation and followed by 14 days treatment with Tan-I. Mice were sacrificed at day 14. B Renal fibrosis was assessed by Masson’s trichrome staining and then quantified. Bars = 100 μm. C The expression of FN, α-SMA, Snail and pSmad3 were analyzed by Western blotting and then quantified. N = 8–11 in each experimental group and one representative of at least three independent experiments is shown. Data represents mean ± SD. *p < 0.05 vs Sham/DMSO. **p < 0.01 vs Sham/DMSO. ***p < 0.001 vs Sham/DMSO ^#p < 0.05 vs UUO/DMSO. ^## p < 0.01 vs UUO/DMSO. ^### p < 0.001 vs UUO/DMSO. Full-length blots are presented in Supplementary Fig. 2